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Petri Dishes

Agile BioScience: Separating Isoforms with Precision – Ion Exchange Chromatography

Ion Exchange Chromatography

Ion exchange chromatography (IEX) is an indispensable tool for protein isoform separation, providing crucial insights into product heterogeneity. Agile BioScience offers advanced IEX services to empower your biopharmaceutical characterization.

Why IEX for Isoform Characterization Matters:

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  • Charge-Based Separation: IEX isolates protein isoforms with subtle differences in surface charge due to post-translational modifications, sequence variations, and more.

  • Product Quality Assessment: Identifies charge variants that can impact efficacy, stability, and safety.

  • Manufacturing Optimization: Monitors process changes that might alter the isoform profile, ensuring batch-to-batch consistency.

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Our Approach

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  • Method Selection: We choose the optimal IEX mode (cation or anion exchange) based on your protein's properties.

  • Gradient Optimization: Carefully designed elution gradients maximize resolution and enable the separation of closely related isoforms.

  • Data-Driven Guidance: Our expert interpretation of IEX results reveals your protein's charge heterogeneity and supports informed decision-making.

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Benefits of Choosing Agile BioScience:

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  • In-Depth Isoform Profiling: Gain comprehensive insights into your protein's charge variants.

  • Expertise You Can Trust: Our scientists are skilled in developing and optimizing IEX methods for complex protein analysis.

  • Actionable Insights: Identify and address potential issues related to your product's isoform distribution.

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Contact us to discover how ion exchange chromatography can bolster your biopharmaceutical development.

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