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Agile BioScience specializes in C-terminal protein sequencing, a technique that reveals the amino acid sequence at the end of a protein chain, called the C-terminus. This method helps understand the structure and function of proteins better.

Edman Degradation 

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​Agile BioScience offers Edman Degradation, a pioneering method developed by Pehr Edman in the 1950s for C-terminal protein sequencing. This technique selectively removes the N-terminal amino acid, allowing inference of the C-terminal amino acid.

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Mass Spectrometry 

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Agile BioScience utilizes Mass Spectrometry as the premier method for C-terminal protein sequencing, leveraging its sensitivity and versatility.

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1. Protein Digestion: The protein undergoes digestion into smaller peptides, typically with enzymes like trypsin, resulting in a mixture of peptide fragments.

2. Peptide Ionization: The peptide mixture is ionized using techniques such as electrospray ionization (ESI) or matrix-assisted laser desorption/ionization (MALDI).

3. Mass Analysis: Ionized peptides are then subjected to mass spectrometry analysis, determining their mass-to-charge ratios.

4. Fragmentation: Tandem mass spectrometry (MS/MS) techniques fragment specific peptides, generating product ions for amino acid sequence determination.

5. Data Analysis: Advanced software tools interpret mass spectra data to deduce the amino acid sequence of the peptides.

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Partner with Agile Bioscience to decode the secrets of your protein's C-terminus. Contact us for a consultation!